Figure 2
From: Secretion-mediated STAT3 activation promotes self-renewal of glioma stem-like cells during hypoxia
HIF-1α mediated STAT3 phosphorylation enhances glioma self-renewal. (a) Western blot analysis of HIF-1α, phospho-STAT3, and total STAT3 in TSC1 cells expressing scramble shRNA or HIF-1α shRNA and incubated in normoxia or hypoxia (16 h). (b) Rescue of TSC1 subsphere formation by STAT3C after inhibition of HIF-1α expression. Data points represent the percentage of plated cells that grew as spheres in three independent experiments conducted in triplicate and are presented as the mean±s.d. (*P<0.05). (c) Rescue of TSC1 colony formation in soft agar by STAT3C after inhibition of HIF-1α expression. Data points represent three independent experiments conducted in triplicate and are presented as the mean±s.d. (*P<0.05).
