Figure 2
From: Selective targeting p53WT lung cancer cells harboring homozygous p53 Arg72 by an inhibitor of CypA
Decreased expression of G3BP1 in A549 cells induced by HL001. (a) The quantitative differences of G3BP1 expression after the treatment of 10 μM HL001 in the 2-DE images through three independent experiments. Bar groups represent the mean±s.d. Student’s t-test was performed, *P<0.05. (b and c) Western blotting suggesting decreased G3BP1 induced by HL001. A549 cells were incubated with 20 μM HL001 for different time (b) or different concentrations of HL001 for 36 h (c), and cell lysates were analyzed by western blotting. (d) Kaplan–Meier survival analysis of G3BP1 in LUAD patients. (e and f) The knockdown of G3BP1 suppresses the proliferation of A549 cells. A549 cells stably expressing control shRNA or G3BP1 shRNA were lysed and subjected to western blotting (e), and the proliferation of A549-shG3BP1 cells were assessed by MTS assay at 24, 48, 72 and 96 h through three independent experiments carried out in triplicate (f). Bar groups represent the mean±s.d. Student’s t-test was performed, **P<0.01. (g–i) The overexpression of G3BP1 produces resistance to the suppression activity of HL001. A549 cells stably expressing G3BP1 were analyzed by western blotting (g), and the proliferation (h) and cell viability (i) of A549-shG3BP1 cells were assessed by MTS assay at 24, 48, 72 and 96 h in the presence of 20 μM HL001 through three independent experiments carried out in triplicate. Bar groups represent the mean±s.d. Student’s t-test was performed, **P<0.01, ***P<0.001.
