Figure 3
From: Selected mitochondrial DNA landscapes activate the SIRT3 axis of the UPRmt to promote metastasis
Metabolic flexibility, activation of the SIRT3 axis of the UPRmt characterizes invasive cell lines and modulation of SOD2 levels alone alters the invasion capacity. (a) OCR/ECAR ratio obtained by Seahorse analysis of the indicated cell lines in three separate experiments; blue indicates a higher use of oxidative phosphorylation over glycolysis and pink indicates a higher use of glycolysis over oxidative phosphorylation; gradient indicates equal use of both. (b) Western blot analysis of markers of the UPRmt in the indicated breast cancer cell lines. (c) Quantification of LC3 and LC3IIB in non-invasive and invasive cell lines. (d) Western blotting of SOD2 levels in a stable clone overexpressing SOD2. (e) (i) Invasion assay at 24 h in MCF-7 and MCF7-SOD2 cells, (ii) a quantification of the invasion. (f) (i) Colony formation assay in MCF-7 and MCF-7-SOD2 cells, and (ii) quantification of colonies. (g) Western blotting of SOD2 in MDA-MB-231 cells where SOD2 was inhibited by two independent shRNA. (h) A quantification of the invasion with and without shRNA against SOD2 is shown. *P<0.05 calculated by analysis of variance and **P<0.01.
