Figure 2
From: SerpinB2 regulates stromal remodelling and local invasion in pancreatic cancer
Fibroblast-derived SerpinB2 is necessary for efficient collagen I matrix contraction. (a) Photographs showing collagen I matrix contraction over 12 days in the presence of either wild-type or SerpinB2−/− MEFs. (b) Changes in area (mm2) of collagen matrices shown in (a) over the 12-day contraction period. (c, d): Maximum projection through 0–80 μm z-stack of SHG signal intensity of collagen I matrices formed with either (c) wild-type or (d) SerpinB2−/− MEFs (e) Quantification of SHG signal intensity within matrices formed by either wild-type or SerpinB2−/− MEFs, inset: mean SHG signal peak. (f–i) SEM ultrastructure analysis showing surface (f, g) or sagittal section (h, i) of collagen I matrices. Values shown are mean±s.e.m. from three separate experiments, statistical analysis performed using an unpaired t-test.
