Figure 4
From: SerpinB2 regulates stromal remodelling and local invasion in pancreatic cancer
Stromal SerpinB2 attenuates PDAC tumour growth in mixed cell allografts. (a) A mixture of MEFs (wild-type or SerpinB2−/−) and PDAC cells (at a 3:1 MEF:PDAC ratio) were inoculated s.c. into nude mice and allowed to grow for 7 days prior to euthanasia and tissue collection. (b) IVIS imaging of MEF-GFP fluorescence; tumour volume quantification (mean±s.e.m., n=5). (c) Ki-67 staining and quantification at both the border and centre of tumours (with insets showing high magnification view). (d) Maximum projection of SHG signal intensity within MEF:PDAC tumours and quantification of SHG signal (inset shows intensity of SHG signal peak). (e) Picrosirius staining (with insets showing high magnification view) and quantification of collagen I/III coverage of tumours. Individual values shown are means from three representative images per tumour, from five animals per group with mean±s.e.m. denoted. Statistical analyses were performed using unpaired t-tests.
