Figure 2
From: Pre-45s rRNA promotes colon cancer and is associated with poor survival of CRC patients
Pre-45s rRNA was essential for supporting the growth of CRC tumor cells. (a) Expression levels of pre-45s rRNA in colon cancer cell and normal colon epithelial cell were evaluated by real-time PCR. Expression of pre-45s rRNA was compared with commercial normal colon tissue. Knockdown of pre-45s rRNA suppressed cell growth. (b) MTT assay was performed in HCT116, HT29 and Caco-2. Colony formation assay was performed in HCT116, HT29 and Caco-2. Represented images were shown. Flow cytometry was used to evaluate the effect of pre-45s rRNA downregulation on cell-cycle arrest in (c) HCT116, (d) HT29 and (e) Caco-2. Representive graphs were shown. (f) Knockdown of pre-45s rRNA favored the interaction between MDM2 and RpL11. Co-immunoprecipitation was used to determine the interaction between MDM2 and RpL11. RpL11 was immunoprecipiated; immunoblot was used to determine the presence of RpL11 and MDM2 in the immunoprecipitant. siRNA of 5 pmol against pre-45s rRNA was used to knockdown the rRNA in cells. β-Actin was used as an internal control for real-time PCR. All data points are expressed as mean±s.d.
