Figure 5
From: Constitutive activation of breast tumor kinase accelerates cell migration and tumor growth in vivo
Transwell assays demonstrating the effect of BRK on cell migration. (a) Migration of BRK stable MDA-MB-231 cell lines expressing GFP-BRK-WT, or constitutively active GF-BRK-YF or GFP alone were evaluated in 24-well transwell polystyrene membrane with 8 μm size pores (See ‘ Materials and methods’). Migrated cells were fixed with paraformaldehyde, stained with crystal violet for 30 min and the number of migrating cells was counted scored in relative units. (b, c) BRK knockdown significantly suppresses migration of BT20 and SKBR breast cancer cell lines. The stable knockdown cells were prepared as described in Figure 4 legend and analyzed for migration by transwell assay as described above. All results are the mean (±s.e.m.) of more than three separate experiments. Statistical analysis *P<0.05, ***P<0.0001.
