Figure 1
From: Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells
Isolation of CD133-positive cells by radiation exposure. (a) Western blotting of CD133 and β-actin in HepG2 and A172 cells at the indicated day of FR. (b) FACS analysis of CD133-positive cells by using phycoerythrin-conjugated anti-CD133 antibodies. Phycoerythrin-conjugated mouse-IgG antibody is used as negative control. (c) The percentage of CD133-positive cells was determined by FACS analysis as shown in b. (d) CD133 positive cells in the colony of HepG2. The colony was made by incubating irradiated cells for 6 days and then immunofluorescence was performed. The percentage of the colony consisting of CD133-positive cells was shown on the right graph. (e) mRNA expression of GADPH, CD133, Albumin, MDR1, GFAP and BCRP1 in parental 0FR cells (lane 1) and 82FR-31NR cells (lane 2). GADPH was used for internal control. (f) Immunofluorescence staining with cytokeratin14 in 0FR and 82FR-31NR cells of HepG2. Nuclei were stained with Hoechst.
