Figure 3
From: Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells
Activation of the AKT/cyclin D1 pathway for efficient DNA repair in 82FR-31NR cells. (a) Western blotting of phosphorylated-AKT-Serine 473 (P-AKT Ser473), AKT, p53, cyclin D1 and β-actin in 0FR and 82FR-31NR cells. (b) Western blotting of γ-H2AX and β-actin in 0FR and 82FR-31NR cells of HepG2 and A172 after 5-Gy irradiation at indicated time points. (c) Western blotting of γ-H2AX and β-actin in 0FR and 82FR-31NR cells of HepG2 and A172 with radiation plus API-2. Cells were treated with 20 μM of API-2 for 24 h and then the drug was removed by washing out before irradiation. (d) Western blotting of γ-H2AX and β-actin in 0FR and 82FR-31NR cells of HepG2 and A172 by control siRNA (siControl) and cyclin D1 siRNA (siCD1) was shown. The amounts of γ-H2AX were normalized by corresponding β-actin level. The values are expressed relative to the untreated control value.
