Figure 5
From: Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells
Suppression of radioresistance in 82FR-31NR cells by targeting cyclin D1/Cdk4. (a) Western blotting of cyclin D1, Cdk4 and β-actin by control siRNA (siControl), cyclin D1 siRNA (siCD1) and Cdk4 siRNA (siCdk4) are shown in the left panel. Western blotting was performed by using cell extracts collected 48 h after transfection. Survival curves of 82FR-31NR cells with siControl (open circle), siCD1 (closed triangle) or siCdk4 (closed square) after irradiation are shown. Cells were irradiated 24 h after transfection. Asterisks indicate significant radiosensitization in 82FR-31NR cells by siCD1 or siCdk4 compared with 82FR-31NR cells with siControl. (b) FACS analyses of apoptosis after 5-Gy irradiation on 82FR-31NR cells with siControl, siCD1 or siCdk4. Cells were irradiated 48 h after transfection and stained with Annexin V and propidium iodide. The percentages of apoptotic cells are shown on the right graph. Asterisk indicates that siCD1 or siCdk4 significantly induced apoptosis in 82FR-31NR cells compared with siControl.
