Figure 2 | Oncogenesis

Figure 2

From: Genome-wide profiling reveals transcriptional repression of MYC as a core component of NR4A tumor suppression in acute myeloid leukemia

Figure 2

Efficient expression of NR4A1 and NR4A3 in human AML cells using direct IVT-RNA electroporation. (a) Flow cytometry analysis reveals high transfection efficiency (93%) of IVT-EGFP into Kasumi-1 cells with minimal increase in cell death 24 h after electroporation as compared with no electroporation control. (b) Fluorescent microscopy images of IVT-EGFP electroporated Kasumi-1 cells. (c) Time-course western blot analysis of Kasumi-1 cells electroporated with EGFP and Luciferase, NR4A1 or NR4A3 IVT-RNAs.

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