Figure 6
Expression level of MTA1 is correlated with the MTA1 promoter methylation in HCC specimens. (a) The HBV-associated HCC tissues obtained from 30 HCC patients.26 qRT–PCR was performed with cDNA prepared from the microdissected non-tumor or cancer regions. The independent data (normal (N) vs nontumor (NT) region and normal vs cancer) and the matched data (nontumor vs cancer) of gene expression was analyzed using Wilcoxon signed rank test and Mann–Whitney test, respectively. (b) Pearson correlation analysis for comparison of mRNA level of MTA1 and DNMT3a/3b gene in six human normal liver and 30 HCC specimens. (c) Methylation status of the CpG island 1 of four normal human livers and five HCC tissues with higher MTA1 expression were analyzed by bisulfite sequencing (left). Pearson correlation analysis for comparison of MTA1 mRNA expression level and percentage of methylation of the CpG island of MTA1 promoter (right).
