Figure 5

Overexpression of miR-203 suppresses keratinocyte proliferation by blocking G1- to S-phase cell cycle transition. (a) Primary human keratinocytes were transfected with a synthetic precursor molecule for miR-203 (pre-miR-203) or scrambled oligos as negative control (control, ctr). Cell proliferation and cell cycle progression was measured by EdU labeling and subsequent cell cycle analysis by fluorescence-activated cell sorting (FACS). Representative FACS plot from three independent experiments. (b) Bars depict mean±s.d. of the three independent experiments: percentage of cells in the G1, S and G2/M phase of the cell cycle and percentage of EdU+ cells are shown. ^***P<0.001, Student's t-test.