Figure 1
From: Pinellia pedatisecta agglutinin interacts with the methylosome and induces cancer cell death
The amino acid sequence and exogenous expression of PPA in human cells. (a) The amino acid sequence of PPA. Underlined amino acid sequences show three MBMs. (b) The exogenous expression of his-PPA in a normal lung cell line WI38 and lung cancer cell lines A549, H460 and H1299. Cells were transfected with pcDNA3.1/His or pcDNA3.1/His-PPA. Cell lysates were analyzed by western blot with antibodies against 6His or Actin. (c) The exogenous expression of his-PPA in liver cancer cell lines PLC or Hep3B. Cells were transfected with pcDNA3.1/His or pcDNA3.1/His-PPA. Cell lysates were analyzed by western blot with antibodies against 6His or Actin. (d) The exogenous expression of FLAG-PPA in H1299 cells. Cells were transfected with pcDNA3/FLAG or pcDNA3/FLAG-PPA. Cell lysates were analyzed by western blot with antibodies against FLAG or Actin.
