Figure 1

Phosphoproteomic screening. (a) Summary of screening and validation approaches demonstrating that specific novel RTKs are important to the in vitro phenotype of metastatic osteosarcoma cell lines. (b) Phospho-RTK antibody arrays simultaneously assayed for the phosphorylation of 42 individual RTKs in the metastatic LM7 and 143B cell lines. Phospho-tyrosine-positive controls are located in duplicate in each corner of the arrays. Each array is representative of three independent experiments.