Figure 2 | Oncogenesis

Figure 2

From: Multiple receptor tyrosine kinases promote the in vitro phenotype of metastatic human osteosarcoma cell lines

Figure 2

Functional genomic screening. (a–e) siRNA screening of 12 RTKs in LM7 cells after mRNA knockdown by siRNA pools. Knockdown efficiency was assessed by real-time PCR measurements of mRNA levels (mean±s.e.m. of three PCR well replicates) (a) The effect of the siRNA pools on the in vitro phenotype was assessed by measuring motility (b), colony formation (c), invasion (d) and cell growth (e). (f–j) siRNA screening of nine RTKs in 143B cells after mRNA knockdown by siRNA pools. The effect of the siRNA pools on the in vitro phenotype was assessed by measuring mRNA expression (f), motility (g), colony formation (h), invasion (i) and cell growth (j). Results (b–e and g–j) are compared with cells transfected with the control non-targeting siRNA pool and presented as the percent of control (mean±s.e.m. of the number of replicates indicated in the methods for each assay). Gray bars represent those siRNA pools that inhibited the indicated assay by ⩾35% (below the horizontal line).

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