Figure 4 | Oncogenesis

Figure 4

From: Multiple receptor tyrosine kinases promote the in vitro phenotype of metastatic human osteosarcoma cell lines

Figure 4

EphB2 contributes to motility and colony formation by the LM7 cell line. (a–c) The first validation strategy compared the effects of the siRNA pool to that of the individual duplexes. Knockdown efficiency was assessed by real-time PCR measurements of EphB2 mRNA levels (mean±s.e.m. of three PCR well replicates) (a). Effects of the siRNAs on in vitro phenotypes were assessed by measuring motility (b), and colony formation (c). Results are presented as mean±s.e.m. of the number of replicates indicated in the Materials and methods for each assay. (d–f) The second validation strategy compared the effects of an antisense LNA/DNA gapmer targeting EphB2 to that of the siRNA pool. Knockdown efficiency by the antisense or siRNA pool was assessed by measuring EphB2 mRNA levels by real-time PCR, comparing levels with cells treated with either the non-targeting antisense LNA/DNA gapmer or the siRNA non-targeting pool (d). Effects of the antisense LNA/DNA gapmer and the siRNA pool on in vitro phenotypes were assessed by motility (e) and colony formation (f). Results are presented as mean±s.e.m. of five independent experiments.

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