Figure 4
From: Regulation of subcellular distribution and oncogenic potential of nucleophosmin by plakoglobin
PG and NPM act synergistically to decrease motility and invasiveness of MDA-231 cells. (a, c) Transwell migration assays were performed in triplicates for MCF-10-2A (control), MDA-231, -231-PG, -231-NPM-KD and -231-NPM-KD+PG cell lines. At 6, 12 and 24 h time points the membranes were fixed, stained, cut, mounted on slides, viewed under an inverted microscope and six random fields counted for each membrane. Histograms represent the average±s.d. of the number of migrated cells for each cell line normalized to that of MDA-231 cells. (b, d) Twenty-four hour invasion assays were performed as described in A with the exception that membranes were coated with Matrigel.
