Table 1 Antibodies, fluorescent probes and their respective dilutions in specific assays
From: Regulation of subcellular distribution and oncogenic potential of nucleophosmin by plakoglobin
Antibodies | Assay | Source/reference | |||
|---|---|---|---|---|---|
IP | WB | IF | IHC | ||
Primaries | |||||
Nucleophosmin (m)a | 1:100 | 1:1000 | Abcam (AB10530) | ||
Nucleophosmin (r)a | 1:50 | Santa Cruz (SC-5564) | |||
Plakoglobin (m) | 1:100 | 1:1000 | 1:200 | 1:400 | Transduction Laboratories (610254) |
Plakoglobin (r) | 1:100 | 1:1000 | Reference18 | ||
E-cadherin (m) | 1:800 | Transduction Laboratories (610182) | |||
β-catenin (r) | 1:1200 | Sigma (C-2206) | |||
Actin (m) | 1:1000 | Sigma (A-4700) | |||
Lamin B1 (r) | 1:1000 | Santa Cruz (SC-20682) | |||
Fibrillarin (m) | 1:1000 | Abcam (AB18380) | |||
Secondaries | |||||
Alexa Fluor 488 | 1:750 | Molecular Probes (A11035) | |||
Anti-mouse HRP | 1:5000 | Sigma (054H-8914) | |||
Anti-rabbit HRP | 1:5000 | Sigma (054H-8918) | |||
