Figure 3 | Oncogenesis

Figure 3

From: CCR7-expressing B16 melanoma cells downregulate interferon-γ-mediated inflammation and increase lymphangiogenesis in the tumor microenvironment

Figure 3

STAT1 expression and CCR7-B16 tumorigenesis. STAT1 protein levels in tumor samples before and after CD45+ cell depletion were evaluated by western blot. Cropped STAT1 and β-actin bands are shown, but a full-length blot is presented in Supplementary Figure 2A (a). CCR7-B16 cells were incubated overnight in the presence or absence of mouse IFN-γ (mIFN-γ; 1 ng/ml) before mouse footpad inoculation. Flow cytometry analysis for STAT1 expression in IFN-γ-treated and non-treated CCR7-B16 cells is shown, with mean fluorescent intensities of 97.75 and 14.6 (b). After inoculation of IFN-γ-treated and non-treated CCR7-B16 cells into the footpads, resulting footpad tumor volumes (mm3) were measured by a caliper on day 24 (c).

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