Figure 2
Loss of Erbin induces STAT3 activation in cervical cancer cells. (a, b) HeLa/NC and HeLa/Erbin-sh cells were cultured in the media containing 1% or 10% or no serum. The phosphorylations of FAK (a) and Src (b) were analyzed by western blot. (c) Parental HeLa (HeLa/WT), HeLa/NC and HeLa/Erbin-sh cells were cultured in the media containing 1% or no serum. The phosphorylation of STAT3 was analyzed by western blot. The relative STAT3 phosphorylation levels were determined by densitometry and normalized with protein levels. (d) HeLa/NC and HeLa/Erbin-sh cells were cultured in the media containing 1% serum in the plates coated with PolyHEMA. The phosphorylation of STAT3 was analyzed at the indicated time points. The relative STAT3 phosphorylation levels were determined by densitometry and normalized with protein levels. (e) HeLa/NC and HeLa/Erbin-sh cells were cultured in serum-free media. The phosphorylation of STAT3 was analyzed at the indicated time points. (f) HeLa/NC and HeLa/Erbin-sh cells were cultured in serum-free media overnight and then treated with 10 μM ISO. The phosphorylation of STAT3 was analyzed at the indicated time points. **P<0.01.
