Figure 1
From: A novel orthotopic mouse model of head and neck cancer and lymph node metastasis
Establishment of the USC-HN3-GFP HNSCC cell line in the xenograft mouse model. (a) Primary tumor growth of USC-HN3-GFP cells in two representative xenograft mice after 4 weeks of growth under fluorescent and bright light microscopy. Flourescent microscopy demonstrated tumor in the tongue (A1, B1) and tumor in the tissue and gland (C1, D1). Bright light microscopy demonstrated tumor in the tongue (A2, B2) and tumor in the tissue and gland (C2, D2). (b) Hematoxylin and eosin staining and IHC of the primary tumor and LN metastasis tissue in the mouse model. Tissue is stained with antibodies against EGFR and Ki-67. Primary tumor and LN demonstrate histological features consistent with SCC. Expression of EGFR and Ki-67 indicates that primary tumor and LN are neoplastic cells with high proliferative index. Negative control was stained without primary antibody.
