Table 2 Colon cancer cell lines classified by the molecular pathways CIN, MSI and CIMP, and mutation status of cancer critical genes

Cell line	MSI status	CIMP panel 1	CIMP panel 2	CIN	KRAS	BRAF	PIK3CA	PTEN	TP53
CO-115	MSI	+	+	−	wt	V600E	wt	E157fs;R233X	wt
DLD-1	MSI	+	+	−⁴⁶	G13D	wt	E545K;D549N	wt	S241F
HCT-116	MSI	+	+	−	G13D	wt	H1047R	wt	wt
HCT-15	MSI	+	+	−	G13D	wt	E545K;D549N	wt	S241F
LoVo	MSI	−	−	−	G13D;A14V	wt	wt	wt	wt
LS-174T	MSI	−	−	−	G12D	wt	H1047R	wt	wt
RKO	MSI	+	+	−	wt	V600E	H1047R	wt	wt
SW48	MSI	+	+	−	wt	wt	wt	wt	wt
TC71	MSI	+	−	−	G12D	wt	wt	R233X	C176Y;R213X
Caco-2	MSS	+	−	+⁴⁸	wt	wt	wt	wt	E204X
COLO 320	MSS	−	−	+	wt	wt	wt	wt	R248W
EB	MSS	−	+	+	G12D	wt	E545K	wt	wt
FRI	MSS	−	−	+	G13D	wt	E545K	wt	C277F
HT-29	MSS	+	+	+	wt	V600E	P449T^b	wt	R273H
IS1	MSS	+	−	+	G12D	wt	wt	wt	Y163H
IS3	MSS	−	−	+	G12D	wt	wt	wt	Y163H
LS1034	MSS	−	−	+	A146T^b	wt	wt	wt	G245S
NCI-H508	MSS	−	−	+⁴⁶	wt	G596R	E545K	wt	R273H
SW1116	MSS	+	−	+⁴⁷	G12A	wt	wt	wt	A159D
SW480	MSS	−	−	+	G12V	wt	wt	wt	R273H;P309S
SW620	MSS	+	−	+⁴⁶	G12V	wt	wt	wt	R273H;P309S
SW948	MSS	−	−	+⁴⁷	Q61L	wt	E542K	wt	G117fs
V9P	MSS	−	−	+	wt	wt	wt	wt	G245D
WiDr	MSS	+	+	+^a	wt	V600E	P449T^b	wt	R273H

Abbreviations: CIN, chromosomal instability pathway; MSI, microsatellite instability; MSS, microsatellite stable; CIMP, CpG island methylator phenotype; X, stop codon; fs, frame shift; wt, wild type.
Mutations are annotated at the protein level as described by den Dunnen et al.⁵⁴ (standard one-letter amino acid abbreviations, X and fs). For further details, see Supplementary Table 1.
^aNo publication on WiDr karyotype was found; however, WiDr and HT-29 are identical cell lines.¹⁹
^bPreviously reported mutations not covered by our assays.

Quick links

Search