Figure 2 | Oncogenesis

Figure 2

From: Involvement of polypyrimidine tract-binding protein (PTBP1) in maintaining breast cancer cell growth and malignant properties

Figure 2

Ectopic expression of PTBP1 promotes AIG of HMEC 184A1 cells. (a) Western blot showing the expression of PTBP1–EGFP fusion protein in 184A1 cells. (b) Cell growth curves determined by MTT assay. (c) Micrographs showing the appearance of parental 184A1 cells, 184A1 cells carrying control vector (184A1/LV), 184A1 cells expressing PTBP1–EGFP (184A1/PTBP1–EGFP) as well as 184AA2 cells. Magnification, × 100. (d) Colony formation assay. Upper panel: sample micrographs showing the colonies formed in the agar matrix. Magnification, × 40. Lower panel: fold differences of colony formation of 184A1/PTBP1–EGFP, 184AA2 and MCF-7 cells relative to the control cells 184A1/LV.

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