Figure 4

Effects of COL1A1 and IFITM1 inhibition on cell proliferation and on neurosphere-initiating capacity. (a) Cell proliferation was measured by using a MTS assay. Each experiment was carried out in replicates of six and repeated twice and expressed as mean±s.d. (b) Inhibition of COL1A1 resulted in a significant (*P<0.05) decrease of neurosphere-initiating cell (NS-IC) frequency whereas inhibition of IFITM1 expression only slightly modified the neurosphere-initiating capacity. Final cell dilutions yielding 37% of negative wells correspond to the dilution at which there is one NS-ICs per well. Each experiment was performed in duplicate and repeated twice and expressed as mean±s.d.