Figure 1
hAPRIL.133 reliably detects human APRIL in the presence of human serum. (a) Schematic representation of the ELISA setup. We coated 96-wells plates with BCMA-Fc, and detected recombinant APRIL with the newly derived anti-APRIL mAbs followed by an horseradish peroxidase-labeled anti-mouse IgG. (b) The selected hAPRIL.133 mAb detects APRIL reliably independent of whether it was serially diluted in PBS–BSA or in human serum. Two standard curves were generated by serial dilutions of human APRIL in PBS+fetal calf serum 10%+human serum 20% (PBS–fetal calf serum–HS) or in PBS+BSA 1% (PBS–BSA).
