Figure 3
Downregulation of Rap1 eliminated the inhibition effects of TRF2 on etoposide-induced ATM activation. (a) TRF2 eukaryotic expression vector was transfected into SGC7901. Expression of ATM, ATM pS1981, γH2AX and p53 pS15 after treated with 20 μg/ml of etoposide for 6 h were detected by western blot. (b) Rap1 siRNA vector and TRF2 eukaryotic expression vector were cotransfected into SGC7901. Expression of ATM, ATM pS1981, γH2AX and p53 pS15 after treated with 20 μg/ml of etoposide for 6 h were detected by western blot. (c) Rap1 siRNA vector was transfected into SGC7901/VCR. Expression of TRF2, Rap1, ATM, ATM pS1981, γH2AX and p53 pS15 after treated with 20 μg/ml of etoposide for 6 h were detected by western blot.
