Figure 4 | Oncogenesis

Figure 4

From: The phosphorylated prodrug FTY720 is a histone deacetylase inhibitor that reactivates ERα expression and enhances hormonal therapy for breast cancer

Figure 4

FTY720 treatment suppresses advanced tumorigenic mammary lesions in HFD-fed PyMT transgenic mice. Female PyMT transgenic mice were fed with a normal diet (ND) or a Western HFD, and were treated daily with saline or FTY720 (1 mg/kg) by gavage starting after weaning. (a) Tumor volumes were determined at the indicated times. (b) Representative images of 10-week-old female PyMTTg mice fed with ND or HFD without or with FTY720. Note the difference in the size of the tumors in the mammary pads. (c) Tumor volumes and weights were determined at 11 weeks. (d, e) Tumor sections were stained with hematoxylin and eosin (H&E), proliferation determined by Ki67 staining, apoptosis by TUNEL and cyclin D1 expression determined by immunohistochemistry. Scale bars: 200 μm and 100 μm, as indicated. (e) Quantification of Ki67- and TUNEL-positive cells. Data are mean±s.e.m. #P<0.05 compared with ND; *P<0.05 compared with HFD.

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