Figure 5 | Oncogenesis

Figure 5

From: The phosphorylated prodrug FTY720 is a histone deacetylase inhibitor that reactivates ERα expression and enhances hormonal therapy for breast cancer

Figure 5

FTY720 treatment reverses HFD-induced loss of estrogen and progesterone receptors in PyMT transgenic mice. Female PyMT transgenic mice were fed with a normal diet (ND) or a Western HFD, and were treated daily with saline or FTY720 (1 mg/kg) by gavage starting after weaning (n=5 each), as indicated. (a, b) Nuclear extracts from tumors were analyzed by western blotting with the indicated antibodies. (c) Representative images of tumor sections immunostained with anti-ERα or anti-H3-K9ac antibodies. Scale bars: 20 μm. (d) HDAC activity in nuclear extracts of tumors was determined and expressed as arbitrary fluorescence units. (e) FTY720 and FTY720-P levels in nuclear extracts of tumors from mice on HFD treated with FTY720 were measured by liquid chromatography, electrospray ionization/tandem mass spectrometry (LC-ESI-MS/MS). (f) ERα, PR and ErbB2 mRNA levels in tumors were quantified by quantitative real-time PCR (QPCR) and normalized to Gapdh. Data are mean±s.e.m. *P<0.05 compared with ND; #P<0.05 compared with HFD.

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