Figure 5
Heparin blocks the effect of MIBC exosomes on cell migration and invasion. (a–c) Urothelial cells were pretreated with heparin (10 μg/ml) for 30 min and then plated for migration on uncoated transwell inserts (a), Col IV-coated inserts (b) or matrigel-coated inserts (c) in 500 μl serum-free media with MIBC exosomes or PBS for migration or invasion assays. (d, e) MIBC exosomes were pretreated with heparin for 30 min. Urothelial cells were plated on uncoated transwell inserts (d) or matrigel-coated inserts (e) in 500 μl serum-free media, and MIBC exosomes (30 μg/ml) or PBS were plated in the bottom chamber. Migration and invasion assays were carried out in triplicate. Graphs represent averages (normalized to the PBS control-treated cells)±s.e.m. *P<0.05, **P<0.01, ***P<0.001, by a paired Student's t-test.
