Figure 2
From: SPINK1 promotes colorectal cancer progression by downregulating Metallothioneins expression
Knockdown of SPINK1 leads to decrease in cell proliferation, invasion and anchorage-independent growth. (a) SPINK1 expression in SPINK1 knockdown WiDr cells (shSPINK1-1, shSPINK1-2, shSPINK1-3, shSPINK1-4, shSPINK1-5 and shSPINK1-6) as compared with shSCRM (Scrambled) WiDr cells. (b) Cell proliferation assay using shSPINK1-1, shSPINK1-3, shSPINK1-6 and shSCRM WiDr cells at the indicated time points. (c) Boyden chamber matrigel invasion assay using shSPINK1-1, shSPINK1-3, shSPINK1-6 and shSCRM WiDr cells. (d) Same experimental cell lines as c, except soft agar assay for anchorage-independent growth. (e) Foci formation assay using shSPINK1-1 and shSCRM WiDr cells. (f) SPINK1-enriched conditioned media (CM) stimulated cell proliferation in YAMC cells measured by colorimetric water-soluble tetrazolium (WST) assay at the indicated time points. (g) Same as f, except MSIE cells were used. (h) Cell invasion measured by Boyden chamber matrigel invasion assay using YAMC and MSIE cells. Error bars represent mean±s.e.m. P-values derived from two-sided Student’s t-test, *P<0.005, **P<0.05.
