Figure 4
From: SPINK1 promotes colorectal cancer progression by downregulating Metallothioneins expression
Knockdown of SPINK1 leads to upregulation of Metallothioneins (MTs) and other important pathways. (a) SPINK1 expression derived from the microarray profiling data using SPINK1 knockdown WiDr cells, shSPINK1-1, shSPINK1-3 and shSPINK1-6 as compared with shSCRM cells. ACTB and GAPDH were used as control. (b) Volcano plot depicting differentially regulated genes by clustering based on probes that were enriched or depleted (P<0.01) in shSPINK1 cells as compared with shSCRM cells. Note: upregulation of several isoforms of Metallothioneins on SPINK1 silencing. (c) Enriched cellular pathways using differentially regulated genes (P<0.05) uploaded onto Ingenuity pathway analysis software. (d) DAVID analysis showing different pathways upregulated in SPINK1-silenced cells.
