Figure 5
From: SPINK1 promotes colorectal cancer progression by downregulating Metallothioneins expression
SPINK1 knockdown in colon cancer cells confer sensitivity to chemotherapeutic drugs. (a and b) In vitro doxorubicin-induced cytotoxicity assay using shSPINK1-1 and shSCRM colon cancer cells. Cells were cultured in the presence of doxorubicin as shown, followed by colorimetric WST assay. IC50 value was calculated by automated fitting of dose–response curves using Logit regression analysis. (c) Percent inhibition in cell proliferation with doxorubicin treatment using shSPINK1-1 and shSCRM WiDr cells. (d) Boyden chamber Matrigel invasion assay using shSPINK1-1 and shSCRM WiDr cells treated with doxorubicin (100 nM). Error bars represent mean±s.e.m. P-values derived from two-sided Student’s t-test, *P<0.05.
