Figure 3
From: FOXO1 inhibits osteosarcoma oncogenesis via Wnt/β-catenin pathway suppression
FOXO1 inhibits growth of osteosarcoma cell lines and suppresses colony formation capacity. (a) Expression of FOXO1ER protein in stably infected MG-63 and U2OS cells was validated by immunoblotting using anti-FOXO1 antibody. Anti-ACTB antibody was used as a loading control. (b) Induction of nuclear translocation of FOXO1ER by 4-OHT was verified by immunofluorescence analysis. (c) Cell lines were plated in six-well plates at a density of 1 × 105 cells per well (day 0). 4-OHT was added every two days at a concentration of 100 nM. Cells were counted with a hemocytometer at days 2, 4 and 6. Cell viability was verified by trypan blue staining. (d) Representative photographs of colony formation of MG-63-FOXO1ER and U2OS-FOXO1ER cells 12 days after plating; the number of cells plated in each well is also indicated. All experiments were repeated at least three times. Colony formation was quantified and presented as mean±s.d.
