Figure 7
From: FOXO1 inhibits osteosarcoma oncogenesis via Wnt/β-catenin pathway suppression
FOXO1 regulates target gene expression. MG-63 and U2-OS cells expressing FOXO1ER or empty vector were treated with 100 nM 4-OHT or vehicle. One day later, cells were harvested, mRNA and protein was prepared. (a) CDKN1B, CyclinD1, TP53INP1, BIM, NOXA and TRAIL mRNA expression was assessed. EV indicates empty vector. The data represent mean±s.d. of least three experiments. (b) The expression of BAX, BCL-2, NOXA and BIM protein was detected by immunoblotting. Anti-ACTB antibody was used as a loading control.
