Figure 3
Hck enhances Gli1-mediated target gene activation. (a, b, c) NIH3T3 cells were infected with lentiviruses expressing Gli1 alone, Hck alone or the two together. Levels of Shh target genes (a) Gli1, (b) Hck and (c) Ptch1 were determined by RT–qPCR. Endogenous Gli1 levels were measured using primers in the 5′-UTR to distinguish this message from that of the exogenous Gli1. Exogenous human Hck levels were measured using primers specific to human genes. (d) Hck kinase activity is required for maximum activation of Gli1. As in a, Gli1-induced expression of endogenous Gli1 was measured in the presence of wild-type Hck or Hck-K269E kinase-dead mutant (Hck-KE). Presented are means plus s.d.; n=3. Statistical analyses were performed using the Student’s t-test; **P<0.01. qPCR, quantitative PCR.
