Table 1 Clinicopathological parameters and human MDM2 SNP309 genotypes in the unselected cohort of RCC patients

From: Homozygous G/G variant of SNP309 in the human MDM2 gene is associated with earlier tumor onset in Caucasian female renal cell carcinoma patients

Clinicopathological parameters

No. of patients

SNP309

P-valuea

 

Total

T/T

T/G

G/G

 

Total

197

63

116

18

 

Gender

    

0.534

 Females

80

22

50

8

 

 Males

117

41

66

10

 

Morphology

    

0.453

 Clear cell

181

61

103

17

 

 Papillary

10

2

8

0

 

 Chromophobe

5

0

4

1

 

 Unknown

1

0

1

0

 

Age (years)

    

0.760

 ⩽67

101

30

61

10

 

 >67

96

33

55

8

 

Age (years)

 Females

80

    

⩽68

43

9

27

7

0.077b

>68

37

13

23

1

0.024 c

 Males

117

   

0.613

⩽67

62

21

37

4

 

>67

55

20

29

6

 

Tumor stage

    

0.346

 pTa

1

0

1

0

 

 pT1

108

32

70

6

 

 pT2

24

7

14

3

 

 pT3

59

22

28

9

 

 pT4

2

0

2

0

 

 Unknown

3

2

1

0

 

Grouped tumor stage

 pTa+pT1+pT2

133

39

85

9

0.082

 pT3+pT4

61

22

30

9

 

 unknown

3

2

1

0

 

Tumor grade

    

0.855

 G1

30

9

19

2

 

 G2

100

29

60

11

 

 G3

62

22

35

5

 

 Unknown

5

3

2

0

 

Grouped tumor grade

 G1+G2

130

38

79

13

0.662

 G3

62

22

35

5

 

 Unknown

5

3

2

0

 
  1. Age groups were separated by the medians (67 years for all RCC patients, 68 years for female RCC patients and 67 years for male RCC patients).
  2. DNA from part of the unselected cohort (n=137) and the age-selected cohort was isolated from kidney specimens of patients who had undergone surgery for RCC. For this purpose, normal kidney tissue of the highest purity possible was needed. Therefore, three experienced uropathologists (AH, SB and VS) marked tumor-free areas on hematoxylin and eosin staining that had been obtained from formalin-fixed and paraffin-embedded tissues. Then, 5 μm sections from the respective specimens were deparaffinized and manually microdissected after 15 s staining by 0.1% methylene blue using the marked hematoxylin and eosin staining as a template. The purity of the obtained tumor-free kidney tissue was near 100%.
  3. DNA from normal kidney tissue (n=137) was isolated using the Maxwell 16 LEV Blood DNA Kit (Promega Corporation Mannheim, Germany) according to the manufacturer’s instructions. Furthermore, we isolated DNA from the blood lymphocytes of additional RCC patients of the unselected cohort (n=60) as previously described.27
  4. To genotype the unselected cohort (n=137) and the age-selected cohort (n=205) for MDM2 SNP309, we used a restriction fragment length polymorphism approach as summarized in Hitzenbichler et al.,28 whereby the unselected cohort was analyzed on a 2.5% agarose/TAE gel instead of a capillary sequencing system. In addition, genotyping of the other part of the unselected cohort (n=60) was performed as described previously.7
  5. aCross tables, χ2-test, significant values are in bold face.
  6. bAll genotypes.
  7. cGenotypes G/G vs T/T.
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