Figure 1 | Oncogenesis

Figure 1

From: Dietary fat overcomes the protective activity of thrombospondin-1 signaling in the ApcMin/+ model of colon cancer

Figure 1

Endogenous thrombospondin-1 limits tumor multiplicity and enhances the survival of ApcMin/+ mice when fed a low-fat diet but not when fed a high-fat diet. WT, Thbs1−/−, ApcMin/+ and Thbs1−/−:ApcMin/+ mice were fed a low-fat (a) or a high-fat western diet (b) beginning at the time of weaning. (a, b) Survival was measured over time and evaluated using Log-rank (Mantel–Cox test) and Grehan–Breslow Wilcoxon test. Equal numbers of male and female mice were included (N=14) for each group. (c, d) Small and large intestines were excised at the time of death. c and d show representative images of lesions (arrows) and quantitation of intestinal lesions. Small intestine (e) and large intestine (f) lesions were counted under a dissecting microscope and confirmed by H&E staining. N=4–5, *P<0.05. (g, h) Representative images of TUNEL staining in small (left) and large intestine of ApcMin/+ and Thbs1−/−:ApcMin/+ mice quantification is presented for small (i) and large intestines (j). N=6–8 *P<0.05.

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