Figure 2

Thrombospondin-1 and Apc regulate amino-acid metabolism. (a) Pie chart depicting metabolites that exhibited significant diet-dependent changes in levels in Thbs1^−/−:Apc^Min/+ mice relative to Apc^Min/+ fed the same diets. (b) Comparison of metabolites showing differential expression in Thbs1^−/−:Apc^Min/+ mice versus Apc^Min/+ mice and those differing in Thbs1^−/− versus WT mice. (c) Relative levels of amino acids in liver of Thbs1^−/−:Apc^Min/+mice versus Apc^Min/+ mice fed low-fat diet or high-fat diet. Polyp risk associated with altered circulating levels of the indicated amino acids (+) is data from Dazard et al.²⁹ Solid red and green cells indicate P<0.05, and shaded cells indicate 0.05<P<0.1. (d) Levels of representative amino acids (tryptophan, serine) in livers of the indicated mice and diet conditions. (e) Levels of the isoleucine catabolite 3-hydroxy-2-ethylpropionate. For the box plots: center horizontal line, median value; +, mean value; box height, limits of upper and lower quartiles; whiskers, max and min of distribution; ○, extreme data point (N=7–8).