Figure 5

Thrombospondin-1 and dietary fat regulate TCA cycle metabolites and 2-hydroxyglutarate in ApcMin/+ mice. WT, Thbs1−/−, ApcMin/+ and Thbs1−/−:ApcMin/+ mice were fed a low-fat or a high-fat western diet at the time of weaning. Livers were excised and flash frozen for metabolomics analysis. (a) Citric acid cycle metabolites and ratios for the indicated metabolites in livers from the respective mice fed low- or high-fat diets. Solid red and green cells indicate P<0.05, and shaded cells indicate 0.05<P<0.1. (b) citrate, (c) isocitrate, (d) 2-hydroxyglutarate levels in livers from mice of the indicated genotypes, N=8.