Figure 4 | Oncogenesis

Figure 4

From: Loss of OLFM4 promotes tumor migration through inducing interleukin-8 expression and predicts lymph node metastasis in early gastric cancer

Figure 4

OLFM4 decreases the migration potential and induces morphologic change in gastric cancer cells. (a) The expression of OLFM4 in wild-type and transfected gastric cancer cells were detected by western blotting. (b) In the upper panel, the effects of OLFM4 overexpression or recombinant OLFM4 (100 ng/ml) on the migration of MGC80-3 and BGC-823 cells were examined. In the lower panel, the effects of OLFM4 siRNA with or without recombinant OLFM4 (100 ng/ml) on the migration of HGC-27 and AGS cells were examined with transwell assay. (c) The effect of OLFM4 depletion on morphologic change in gastric cancer cells. (d) The effects of OLFM4 siRNA on the viability of HGC-27 and AGS cells were examined with CCK-8 assay. (e) The effect of OLFM4 siRNA on the cell cycle of HGC-27 and AGS cells were detected by flow cytometry assay. In (b–e), experiments were repeated three times. In (b), error bars indicate means±s.e.m. In (c) and (e), images are representative of three independent experiments. N.S, not significant; ***P<0.001; rOLFM4, recombinant OLFM4.

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