Figure 1 | Oncogenesis

Figure 1

From: Loss of the oncogenic phosphatase PRL-3 promotes a TNF-R1 feedback loop that mediates triple-negative breast cancer growth

Figure 1

Knockdown of PRL-3 induces senescence in TNBC cell lines. (a) Phase contrast images of BT-20 and MDA-MB-468 cells transduced with lentivirus to knockdown PRL-3 (shPRL-3 #1) or non-silencing control (pLKO). (b) Quantification of (a) as determined by ImageJ analysis. (c) Phase contrast images depicting SA-β-Gal-positive cells after PRL-3 knockdown in BT-20 and MDA-MB-468 TNBC cells. (d) Each bar depicts the effect of increased serum concentration (20% fetal bovine serum (FBS) vs 10% FBS) on cellular growth as assessed by MTS assay on PRL-3 knockdown, SA-β-Gal-positive clones (red bars). Control (pLKO) cell growth in depicted by blue bars. Images captured at × 10 magnification. Data represented are the mean±s.d. of three independent experiments. *P<0.05 as determined by Student t-test on final data point.

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