Figure 1
From: FLASH protects ZEB1 from degradation and supports cancer cells' epithelial-to-mesenchymal transition
FLASH controls E-cadherin expression through a conserved mechanism in multiple cell lines. (a) HeLa 229, MDA-MB-231, PANC-1 and PC-3M cells were transfected with a pool of siRNA duplexes targeting FLASH. FLASH (top panel) and E-cadherin (middle panel) protein levels in siRNA- and mock-transfected cells were determined by western blot analysis and the expression normalized to actin loading control (bottom panel). (b) Relative mRNA levels of FLASH (top graphs) and E-cadherin (CDH1) (bottom graphs) as determined by quantitative PCR. The graphs represent the average of three independent experiments. The significance of differences was determined by Student's t-test (**P<0.0025).
