Figure 3

ZEB1 protein half-life is reduced in FLASH-depleted cells. (a) HeLa 229 cells were transfected with a pool of siRNA duplexes targeting FLASH and treated with cycloheximide alone (10 μm) or cycloheximide and MG132 (10 μm) for the indicated periods of time. ZEB1 expression was determined by western blot (top panel) and normalized to actin loading control (bottom panel). (b) Expression of ZEB1 after prolonged incubation with cycloheximide in FLASH-depleted and mock-transfected cells was determined by western blot analysis (top panel) and normalized to actin loading control (bottom panel).