Figure 6
ARHGEF5 is required for tumor growth from HCT116 cells that have undergone EMT. (a) Mock and ARHGEF5 KD HCT116 cells were treated with TNF-α for 72 h, and expression of the indicated EMT markers was analyzed by western blotting (lower panels). (b) Mock and ARHGEF5 KD HCT116 cells treated with TNF-α were subjected to soft agar colony formation assays. Values represent the mean±s.d. (n=3, ***P<0.001). (c) Mock and ARHGEF5 KD HCT116 cells were stably transfected with or without myc-tagged Slug, and cell morphology observed (upper). Scale bar: 100 μm. The levels of the indicated EMT markers were analyzed by western blotting (lower). (d) HCT116 cells transfected with the indicated constructs were subjected to soft agar colony formation assays. Values represent the mean±s.d. (n=3, *P<0.05).
