Figure 4

Decreased SUSD2 levels increased migration of HGSOC cells. (a) Boyden chamber analysis. Uncoated Boyden chambers were used to analyze migration of HGSOC cells (OVCAR3, OVSAHO and KURAMOCHI cell lines). Cells that migrated through the chambers were stained with crystal violet and quantitated, as represented in the bar graphs in the top panel. Images of stained OVCAR3 and KURAMOCHI cells were taken 24 h after seeding the experiment. Images of stained OVSAHO cells were taken 48 h after seeding the experiment. Representative images of the experimental outcomes from both the NT and most efficient SUSD2-KD HGSOC cell line model are displayed in the lower panel (images were viewed with a × 10 objective using bright field light microscopy). All migration experiments were performed in triplicate. (b) Wound-healing analysis. Migration of HGSOC cell lines was investigated using a wound-healing assay. The graphs in the top panel show the total area occupied by the designated HGSOC cell line at different time points throughout the experiment. Representative images are displayed in the lower panel of the figure (images were viewed with a × 10 objective using bright field light microscopy). The area occupied by the invading HGSOC cells was calculated using arbitrary units (a.u.) derived from the ImageJ software analysis. All experiments were performed in triplicate. P-values were derived using a standard two-tailed t-test between SUSD2-KD cells and NT controls.