Figure 6

Decreased SUSD2 levels increased protein expression of N-cadherin. (a) Western immunoblot analysis of N- and E-cadherin proteins in HGSOC cells (OVCAR3, OVSAHO and KURAMOCHI cell lines). Proteins from whole-cell lysate were harvested from HGSOC cell line monolayers and OVCAR3 spheroids. The bold numbers shown below the E- and N-cadherin bands represent the fold change in the protein level of E- and N-cadherin, respectively, relative to the NT HGSOC cell line assayed. Fold changes in OVCAR3, OVSAHO and KURAMOCHI cell lines are relative to protein levels in the corresponding NT cell line. (b) Quantification of N-cadherin/E-cadherin in HGSOC cell lines. The bar graphs depict the ratio of N-cadherin/E-cadherin detected in western immunoblot analysis. When calculating protein values of N-/E-, N- and E-cadherin expression were initially normalized to GAPDH protein levels. P-values were derived using a standard two-tailed t-test between the SUSD2-KD protein lysates (monolayers and spheroids as indicated) and corresponding NT lysate values.