Figure 4
Addition of myr-p110α allele into p53fl/fl;KrasG12D double mutants modifies tumor histology in a copy number-dependent manner. (a) Representative hematoxylin and eosin (H&E) sections of mammary tumors from p53fl/fl;KrasG12D, p53fl/fl;KrasG12D;myr-p110αwt/fl and p53fl/fl;KrasG12D;myr-p110αfl/fl mice showing increasing epithelial differentiation with addition of myr-p110α allele. Scale bar represents 0.5 mm and indicates scale for all images. (b) Comparison of histologic and immunohistochemical (CK8, ERα, PR and vimentin) phenotypes between p53fl/fl;KrasG12D and p53fl/fl;KrasG12D;myr-p110αfl/fl tumors. Representative H&E sections show solid sheets and fascicles of pleomorphic spindle cells in p53fl/fl;KrasG12D tumor compared to the epithelial nests and glands present in p53fl/fl;KrasG12D;myr-p110αfl/fl tumor. Both tumors are immunoreactive with CK8 and ERα; the p53fl/fl;KrasG12D tumor is PR negative and vimentin positive, whereas the p53fl/fl;KrasG12D;myr-p110αfl/fl tumor is PR positive and vimentin negative. Scale bar represents 100 μm. Magnification is × 40 (a), × 400 (ERα staining of the p53fl/fl;KrasG12D tumor in b) or × 200 (all remaining images in b).
