Figure 6
Addition of one copy of myr-p110α into p53fl/fl;KrasG12D double mutants accelerates tumor development in lung. (a) Hematoxylin and eosin (H&E) staining of lungs dissected from p53fl/fl;KrasG12D and p53fl/fl;KrasG12D;myr-p110αwt/fl mice 52 days post Ad-Cre virus injection. Pictures of H&E staining were taken with × 40 magnification and are representative. Scale bar represents 1 mm and indicates scale for all images. (b) Bar graphs describing the weight of lung divided by body weight, 52 days after Ad-Cre virus injection. Error bars are the mean±s.e.m. Two-tailed unpaired Student’s t-test was used for statistical analysis. ***P<0.001. (c) Kaplan–Meier survival curve scoring mice showing symptoms from lung tumor growth defined as any symptoms associated with illness. Log-rank (Mantel–Cox) test was used for statistical analysis. ***P<0.001. Data in b and c are representative of three independent experiments using n=5–6 per group.
