Figure 6 | Oncogenesis

Figure 6

From: Notch3 inhibits epithelial–mesenchymal transition by activating Kibra-mediated Hippo/YAP signaling in breast cancer epithelial cells

Figure 6

Confirming the ectopic overexpression and knockdown efficiency of Kibra. (a) The Kibra overexpression construct was engineered, and its successful transfection is shown by GFP expression. (b) RT-PCR confirms that the expression of Kibra mRNA is upregulated after MDA-MB-231 cells are transfected with pCMV plasmids. (c) Western blotting confirms that the expression of Kibra protein is upregulated after MDA-MB-231 cells are transfected with pCMV plasmids. (d) The Kibra knockdown construct was engineered and its successful transfection is shown by GFP expression. (e) RT-PCR confirms that the expression of Kibra mRNA is downregulated after MDA-MB-231 cells are transfected with each of the four knockdown plasmids, shKibra #5–#8. (f) Western blotting shows that Kibra protein in MDA-MB-231 cells is reduced after each of the four knockdown plasmids is transfected. Data are presented as the mean±s.d. of three experiments. *P<0.05, **P<0.01 and ***P<0.001 (Student’s t-test) as compared with controls.

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